*************************************************** * Finite Element Fit Analysis * *************************************************** Data Report for Run "demo1", Cell 1, Wavelength 1 Detailed Run Information: Cell Description: lysozyme / 208-1 mixed in 0.2M NaCl/TE Raw Data Directory: /auc/data/demo1 Rotor Speed: 42000 rpm Average Temperature: 20 ºC Temperature Variation: Within Tolerance Time Correction: 1 minute(s) 12 second(s) Run Duration: 3 hour(s) 3 minute(s) Wavelength: 259 nm Baseline Absorbance: -0.0411945 OD (fitted) Meniscus Position: 6.019 cm (fixed) Slope Error Correction(r): 0 OD (fixed) Slope Error Correction(c,r): 0 OD (fixed) Edited Data starts at: 6.042 cm Edited Data stops at: 7.029 cm Hydrodynamic Settings: Viscosity correction: 1.0266 Viscosity (absolute): 1.0266 Density correction: 1.0081 g/ccm Density (absolute): 1.0081 g/ccm Data Analysis Settings: Analyzed Boundary: 100 % Boundary Position: 0 % Fitting Information: Variance: 2.31524e-05 Standard Deviation 0.00481169 # of Iterations: 40 # of Function Evals.: 105 # of fitted Parameters: 7 # of fitted Data Points: 19740 Component Information: Component 1: Molecular Weight: 139917 Dalton (fitted) Sed. Coeff. (s20,W): 5.46294e-13 s (fitted) Sedimentation Coeff.: 5.27009e-13 s (fitted) Diff. Coeff. (D20,W): 2.06407e-07 D (fitted) Diffusion Coeff.: 2.01449e-07 D (fitted) Partial Concentration: 0.404147 OD (fitted) Partial Spec. Vol. (vbar): 0.54 ccm/g (fixed) vbar (Corr. for 20ºC): 0.54 ccm/g (fixed) Conc. Dep. of s (Sigma): 0 (fixed) Conc. Dep. of D (Delta): 0 (fixed) Buoyancy (Water, 20ºC): 0.460954 Buoyancy (absolute): 0.455626 Hydrodynamic Modeling Information: Radius of Minimal Sphere: 31.0559 Å Radius of Stoke's Sphere: 101.311 Å Frictional Coefficient: 1.96046e-07 Minimal Sphere F0: 5.8656e-08 Frictional Ratio (F/F0): 3.34229 Volume of Molecule: 125464 Å^3 Possible Hydrodynamic Models for this Molecule (hypothetical only!): Prolate Ellipsoid: Length of Semi-Major Axis: 506.716 Å Length of Semi-Minor Axis: 7.68835 Å Axial Ratio: 65.907 Oblate Ellipsoid: Length of Semi-Major Axis: 162.326 Å Length of Semi-Minor Axis: 1.13673 Å Axial Ratio: 142.801 Long Rod: Length of Semi-Major Axis: 491.987 Å Length of Semi-Minor Axis: 6.37079 Å Axial Ratio: 77.2253 Component 2: Molecular Weight: 12120 Dalton (fitted) Sed. Coeff. (s20,W): 1.68584e-13 s (fitted) Sedimentation Coeff.: 1.60537e-13 s (fitted) Diff. Coeff. (D20,W): 1.17221e-06 D (fitted) Diffusion Coeff.: 1.14405e-06 D (fitted) Partial Concentration: 0.336126 OD (fitted) Partial Spec. Vol. (vbar): 0.7121 ccm/g (fixed) vbar (Corr. for 20ºC): 0.7121 ccm/g (fixed) Conc. Dep. of s (Sigma): 0 (fixed) Conc. Dep. of D (Delta): 0 (fixed) Buoyancy (Water, 20ºC): 0.289158 Buoyancy (absolute): 0.282132 Hydrodynamic Modeling Information: Radius of Minimal Sphere: 15.0685 Å Radius of Stoke's Sphere: 17.8392 Å Frictional Coefficient: 3.45205e-08 Minimal Sphere F0: 2.84603e-08 Frictional Ratio (F/F0): 1.21294 Volume of Molecule: 14331.8 Å^3 Possible Hydrodynamic Models for this Molecule (hypothetical only!): Prolate Ellipsoid: Length of Semi-Major Axis: 40.755 Å Length of Semi-Minor Axis: 9.16253 Å Axial Ratio: 4.44801 Oblate Ellipsoid: Length of Semi-Major Axis: 25.4387 Å Length of Semi-Minor Axis: 5.28714 Å Axial Ratio: 4.81144 Long Rod: F/F0 < 1.32, this model is not applicable Scan Information: Scan: Corrected Time: Plateau Concentration: 1: 48 min 36 sec 0.673682 OD 2: 55 min 43 sec 0.670245 OD 3: 63 min 2 sec 0.667991 OD 4: 69 min 56 sec 0.664045 OD 5: 76 min 60 sec 0.658982 OD 6: 83 min 52 sec 0.653 OD 7: 90 min 49 sec 0.651382 OD 8: 98 min 2 sec 0.647027 OD 9: 105 min 6 sec 0.644064 OD 10: 112 min 1 sec 0.639436 OD 11: 118 min 59 sec 0.635255 OD 12: 126 min 15 sec 0.631564 OD 13: 133 min 18 sec 0.628864 OD 14: 140 min 17 sec 0.627873 OD 15: 147 min 14 sec 0.622545 OD 16: 154 min 14 sec 0.6225 OD 17: 161 min 18 sec 0.618682 OD 18: 168 min 27 sec 0.615191 OD 19: 175 min 41 sec 0.609982 OD 20: 182 min 55 sec 0.605127 OD